VU Struct

Welcome to VUstruct!

VUstruct predicts variant pathogenicity for your ‘case’ of interest, in context of 3D protein structure.

VUstruct Pipeline Case Input Form

Below input (upload) either:

Genomic coordinates in VCF, Vanderbilt, or WUSTL format (from which missense variants are automatically determined)

or:

Comma-delimited list of Uniprot Sequence IDs alongside missense amino acid variants of interest. (Genomic conversion is not performed)

Case Label(Required)

Enter an ID such as UDN12435678

Data Format(Required)

Select a format for your variants input.

Vanderbilt-specific Spreadsheet Upload(Required)

Accepted file types: xlsx, Max. file size: 255 MB.

This upload only supports Vanderbilt’s unique UDN variant spreadsheet. To proceed, click [Choose File] and select a .xlsx file from your computer.

Washingto U.-specific Spreadsheet Upload(Required)

Accepted file types: xlsx, Max. file size: 255 MB.

This upload only supports Wash U’s unique UDN variant spreadsheet. To proceed, click [Choose File] and select a .xlsx file from your computer.

VCF Upload (GRCh38 coordinates)

Accepted file types: vcf, vcf, Max. file size: 255 MB.

VCF is a tab-delimited format. Your file will be filtered for missense variants using the Ensembl VEP Your VCF file tab-delimited headers should be minimally: #CHROM POS ID REF ALT

VCF Upload (GRCh37 coordinates to liftover)

Accepted file types: vcf, Max. file size: 255 MB.

VCF is a tab-delimited format. Your GRCh37 file will be first “lifted over” to GRCh38 with the UCSF tool. Then, it will be filtered for missense variants using the Ensembl VEP Your VCF file tab-delimited headers should be minimally: #CHROM POS ID REF ALT

Comma Delimited Variants

Paste a comma delimited set of data…