Rosetta Antibody Workshop 2017

In conjunction with the BSDF conference at Vanderbilt, a workshop focusing on membrane proteins was held in April of 2017. Based on Rosetta 3.8, this workshop included hands-on tutorials for RosettaAntibody structure prediction, HCDR3 loop modeling, computational affinity maturation, antibody-antigen docking, epitope grafting onto scaffolds, and electron density refinement, as well as presentations on Rosetta basics.

Zip file (93 MB) of all presentations and tutorials for the 2017 workshop

Main Topics:

Tutorial 1: RosettaAntibody Structure Prediction

• Presentation (pdf)
• Tutorial (pdf)
• Tutorial Materials (zip)

Tutorial 2: HCDR3 Loop Modeling

• Presentation (pdf)
• Tutorial (pdf)
• Tutorial Materials (zip)

Tutorial 3: Design & Computational Affinity Maturation

• Presentation (pdf)
• Tutorial (pdf)
• Tutorial Materials (zip)

Tutorial 4: Epitope Grafting

• Presentation (pdf)
• Tutorial (pdf)
• Tutorial (zip)

Tutorial 5: Antibody-Antigen Docking

• Presentation (pdf)
• Tutorial (pdf)
• Tutorial Materials (zip)

Tutorial 6: Refinement into Electron Density

• Tutorial by Dr. Frank Dimaio of the University of Washington

Short Talk Topics:

Tutorial 1: Introduction to Antibodies

• Presentation (pdf)

Tutorial 2: Rosetta I/O

• Presentation (pdf)

Tutorial 3: Rosetta Energy Function

• Presentation (pdf)

Tutorial 4: RosettaScripts XML

• Presentation (pdf)

Tutorial 5: Using Experimental Data with Rosetta

• Presentation (pdf)